Pyelonephritis, in which Staphylococcus intermedius was isolated, was diagnosed in a 4-year-old female Siberian tiger (Panthera tigris altaica). At necropsy, the renal pelvis was dilated with a large amount of purulent exudates. Microscopically, the lesions of renal pelvis and medulla consisted of necrotic foci intermingled primarily with numerous degenerative neutrophils and a few lymphocytes, plasma cells and macrophages. Bacteriology showed the presence of S. intermedius. This is the first report on pyelonephritis associated with S. intermedius in wild felidae.