Improved analytical sensitivity reveals the occurrence of gender-related variability in diamine oxidase enzyme activity in healthy individuals

Elena García-Martín; Pedro Ayuso; Carmen Martínez; José A G Agúndez

doi:10.1016/j.clinbiochem.2007.07.019

Improved analytical sensitivity reveals the occurrence of gender-related variability in diamine oxidase enzyme activity in healthy individuals

Clin Biochem. 2007 Nov;40(16-17):1339-41. doi: 10.1016/j.clinbiochem.2007.07.019. Epub 2007 Aug 11.

Authors

Elena García-Martín¹, Pedro Ayuso, Carmen Martínez, José A G Agúndez

Affiliation

¹ Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Extremadura, Avda. de Elvas s/n, 06071-Badajoz, Spain. elenag@unex.es

PMID: 17826755
DOI: 10.1016/j.clinbiochem.2007.07.019

Abstract

Objectives: Serum diamine oxidase (DAO; EC 1.4.3.6) activity is often employed as a clinical indicator of the integrity of intestinal mucosa. However, interindividual variation in enzyme activity and reference values for healthy women and men have not been studied.

Design and methods: DAO activity was measured by using cadaverine coupled to O-dianisidine oxidation in 50 healthy individuals.

Results: The mean activity was 7.59+/-3.67 U/L for women and 2.38+/-0.71 U/L for men (p<0.001).

Conclusions: Gender is a major determinant for DAO activity in healthy subjects.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Amine Oxidase (Copper-Containing) / blood*
Amine Oxidase (Copper-Containing) / metabolism
Cadaverine / metabolism
Dianisidine / metabolism
Female
Humans
Male
Oxidation-Reduction
Sex Factors

Substances

Amine Oxidase (Copper-Containing)
Cadaverine
Dianisidine