Comparative analysis of mutants in the mycothiol biosynthesis pathway in Mycobacterium smegmatis

Biochem Biophys Res Commun. 2007 Nov 9;363(1):71-6. doi: 10.1016/j.bbrc.2007.08.142. Epub 2007 Aug 31.

Abstract

The role of mycothiol in mycobacteria was examined by comparative analysis of mutants disrupted in the four known genes encoding the protein machinery needed for mycothiol biosynthesis. These mutants were sensitive to acid stress, antibiotic stress, alkylating stress, and oxidative stress indicating that mycothiol and mycothiol-dependent enzymes protect the mycobacterial cell against attack from various different types of stresses and toxic agents.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / genetics
  • Acetyltransferases / metabolism*
  • Cysteine / biosynthesis*
  • Glycopeptides / biosynthesis*
  • Inositol / biosynthesis*
  • Mutation
  • Mycobacterium smegmatis / genetics*
  • Mycobacterium smegmatis / metabolism*
  • NADH, NADPH Oxidoreductases / genetics
  • NADH, NADPH Oxidoreductases / metabolism*
  • Signal Transduction / physiology*

Substances

  • Glycopeptides
  • mycothiol
  • Inositol
  • NADH, NADPH Oxidoreductases
  • disulfide reductase, mycothiol
  • Acetyltransferases
  • acetyl-CoA 1-D-myo-inosityl 2-L-cysteinylamido-2-deoxy-alpha-D-glucopyranoside acetyltransferase
  • Cysteine