DNA binding and nuclease activity of a one-dimensional heterometallic nitrosyl complex

Int J Biol Macromol. 2007 Dec 1;41(5):579-83. doi: 10.1016/j.ijbiomac.2007.07.016. Epub 2007 Jul 28.

Abstract

The interaction of a structurally characterized Sr-Fe nitrosyl complex with DNA has been studied by UV-vis and fluorescence spectroscopy, viscometric, and gel electrophoresis techniques. From the absorption titration studies the intrinsic binding constant of the complex with DNA was calculated to be 1.6x10(4)M(-1). Fluorimetric studies indicate that the complex compete with EB in binding to DNA. The complex shows nuclease activity on pUC19 supercoiled DNA in presence of H(2)O(2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography, X-Ray
  • DNA / chemistry*
  • DNA / metabolism*
  • Deoxyribonucleases / chemistry*
  • Deoxyribonucleases / metabolism*
  • Electronics
  • Hydrogen Peroxide
  • Models, Molecular
  • Nitrogen Oxides / metabolism
  • Protein Conformation
  • Spectrophotometry
  • Spectrophotometry, Ultraviolet

Substances

  • Nitrogen Oxides
  • DNA
  • Hydrogen Peroxide
  • Deoxyribonucleases