[Screening of polypeptides interacting with Rel homology domain of NF-kappaB p50 subunit]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Sep;23(9):804-6.
[Article in Chinese]

Abstract

Aim: To obtain the polypeptides interacting with NF-kappaB p50 Rel homology domain (RHD) by yeast two-hybrid technique.

Methods: Using NF-kappaB p50 RHD as bait, the polypeptides interacting with NF-kappaB p50 RHD were screened from a 16-peptide cDNA library by yeast two-hybrid technique. The false positive clones were screened out but the positive clone were identified by beta-GAL assay, yeast mating, and one to one yeast two-hybrid method.

Results: 8 positive clones were obtained. The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank.

Conclusion: 8 novel polypeptides interacting with NF-kappaB p50 RHD were obtained, but their functions need to be validated in further experiments.

Publication types

  • English Abstract

MeSH terms

  • Amino Acid Sequence
  • Databases, Genetic
  • Genes, Reporter
  • Lac Operon
  • Molecular Sequence Data
  • NF-kappa B p50 Subunit / chemistry*
  • NF-kappa B p50 Subunit / metabolism*
  • Peptides / analysis
  • Peptides / chemistry
  • Peptides / metabolism*
  • Plasmids / analysis
  • Plasmids / genetics
  • Plasmids / metabolism
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid*
  • Transcription Factor RelA / metabolism*
  • Transcription Factor RelB / metabolism*
  • Two-Hybrid System Techniques

Substances

  • NF-kappa B p50 Subunit
  • Peptides
  • Transcription Factor RelA
  • Transcription Factor RelB