Aim: To study the effects of Fumonisin B1(FB1) on HLA-I on human peripheral blood mononuclear cells (PBMC) in vitro.
Methods: The expression of HLA-I on PBMC by FB1 pretreatment at different dosage(10, 50 micromol/L) was detected with flow cytometry (FCM), Western blot, and semi-RT-PCR, respectively.
Results: The expression of HLA-I on PBMC in vitro at the two experimental concentration was both lower than that of the control after FB1 treatment for 24 h as represented by fluorescence intensity by FCM analysis. Western blot results further confirmed the above results. At mRNA level, HLA-A, B and C mRNA were detected by RT-PCR, and the results showed that no changes were found on the expression of HLA-A, B mRNA between FB1 treated group and the control group, but HLA-C mRNA was inhibited in FB1 treated groups.
Conclusion: 10 and 50 micromol/L FB1 could inhibit the expression of HLA-I on human PBMC in vitro at 24 h treatment.