[Effects of fumonisin on HLA-I expression on human peripheral blood mononuclear cells in vitro]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Sep;23(9):794-6.
[Article in Chinese]

Abstract

Aim: To study the effects of Fumonisin B1(FB1) on HLA-I on human peripheral blood mononuclear cells (PBMC) in vitro.

Methods: The expression of HLA-I on PBMC by FB1 pretreatment at different dosage(10, 50 micromol/L) was detected with flow cytometry (FCM), Western blot, and semi-RT-PCR, respectively.

Results: The expression of HLA-I on PBMC in vitro at the two experimental concentration was both lower than that of the control after FB1 treatment for 24 h as represented by fluorescence intensity by FCM analysis. Western blot results further confirmed the above results. At mRNA level, HLA-A, B and C mRNA were detected by RT-PCR, and the results showed that no changes were found on the expression of HLA-A, B mRNA between FB1 treated group and the control group, but HLA-C mRNA was inhibited in FB1 treated groups.

Conclusion: 10 and 50 micromol/L FB1 could inhibit the expression of HLA-I on human PBMC in vitro at 24 h treatment.

MeSH terms

  • Animals
  • Blotting, Western
  • Flow Cytometry
  • Fumonisins / pharmacology*
  • Gene Expression Regulation / drug effects*
  • Histocompatibility Antigens Class I / genetics*
  • Histocompatibility Antigens Class I / metabolism*
  • Humans
  • Leukocytes, Mononuclear / drug effects*
  • Leukocytes, Mononuclear / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Fumonisins
  • Histocompatibility Antigens Class I
  • RNA, Messenger
  • fumonisin B1