Abstract
Gastrodia elata Blume (GEB) is an important medicinal plant in Korea. In order to confirm the anti-tumor activities of GEB extracts, we carried out various in vitro anti-tumor assays, including a wound assay and an invasion assay using an ethyl ether extract of GEB. The results showed that the GEB extract exhibits potent anti-tumor activity in vitro in a dose-dependent manner. The expression of CD44, cdc42, Timp-2 or RhoA mRNA did not change by GEB treatment, compared to that of the control. GTP-Ras, an active form of a G-coupled protein family, however, is associated with the anti-tumor activity of GEB extracts. We examined various molecular markers related to metastasis by reverse transcriptase-polymerase chain reaction with the extract of GEB-treated B16 cells. There was an increase in GTP-Ras expression by the Gastrodia elata Blume extract. Together, these results suggest that the Gastrodia elata Blume extract could have potential in alleviating tumorigenesis, by a GTP-Ras-dependent pathway; although the precise molecular mechanisms are still being examined.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cells, Cultured / drug effects
-
Cells, Cultured / metabolism
-
Collagen
-
Drug Combinations
-
Endothelium, Vascular / cytology
-
Endothelium, Vascular / drug effects
-
Endothelium, Vascular / metabolism
-
Gastrodia / chemistry*
-
Hyaluronan Receptors / genetics
-
Hyaluronan Receptors / metabolism
-
Laminin
-
Medicine, East Asian Traditional
-
Melanoma, Experimental / drug therapy*
-
Melanoma, Experimental / metabolism
-
Melanoma, Experimental / pathology
-
Mice
-
Neoplasm Invasiveness / pathology
-
Phytotherapy
-
Plant Extracts / pharmacology*
-
Proteoglycans
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Reverse Transcriptase Polymerase Chain Reaction
-
Signal Transduction / drug effects*
-
Tissue Inhibitor of Metalloproteinase-2 / genetics
-
Tissue Inhibitor of Metalloproteinase-2 / metabolism
-
Umbilical Veins / cytology
-
Umbilical Veins / drug effects
-
Umbilical Veins / metabolism
-
Wound Healing / drug effects
-
cdc42 GTP-Binding Protein / genetics
-
cdc42 GTP-Binding Protein / metabolism
-
ras Proteins / genetics
-
ras Proteins / metabolism*
-
rhoA GTP-Binding Protein / genetics
-
rhoA GTP-Binding Protein / metabolism*
Substances
-
Drug Combinations
-
Hyaluronan Receptors
-
Laminin
-
Plant Extracts
-
Proteoglycans
-
RNA, Messenger
-
matrigel
-
Tissue Inhibitor of Metalloproteinase-2
-
Collagen
-
cdc42 GTP-Binding Protein
-
ras Proteins
-
rhoA GTP-Binding Protein