A questionnaire survey was performed to evaluate perceived organizational well-being of nurses in several prominent hospitals in Rome (Italy). The term "organizational well-being" refers to all of the organizational processes and practices that stimulate the dynamics of working relationships leading to the promotion and improvement of quality of life, and of the degree of physical, psychological and social well-being in the work environment. In total 853 nurses from several hospitals in Rome participated in the survey. Results show on one hand, mainly satisfactory nursing working conditions, but on the other, a lack of professional fulfilment, no influence by nurses in the decision-making processes, and a lack of career development opportunities, all of which lead to a total mistrust toward the hospital management.