Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria

Christian U Riedel; Pat G Casey; Heidi Mulcahy; Fergal O'Gara; Cormac G M Gahan; Colin Hill

doi:10.1128/AEM.01394-07

Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria

Appl Environ Microbiol. 2007 Nov;73(21):7092-5. doi: 10.1128/AEM.01394-07. Epub 2007 Aug 31.

Authors

Christian U Riedel¹, Pat G Casey, Heidi Mulcahy, Fergal O'Gara, Cormac G M Gahan, Colin Hill

Affiliation

¹ Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland.

Abstract

A novel vector has been constructed for the constitutive luminescent tagging of gram-negative bacteria by site-specific integration into the 16S locus of the bacterial chromosome. A number of gram-negative pathogens were successfully tagged using this vector, and the system was validated during murine infections of living animals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromosomes, Bacterial
Genetic Vectors*
Gram-Negative Bacteria / genetics*
Gram-Negative Bacteria / isolation & purification
Luminescent Proteins / metabolism*
Mice
RNA, Ribosomal, 16S / metabolism
Recombinant Proteins

Substances

Luminescent Proteins
RNA, Ribosomal, 16S
Recombinant Proteins