Fungus-growing ants (Attini) are in reality unique fungus-culturing insects.There are several hundred species in some dozen genera, of which Acromyrmex and Atta are the conspicuous leaf-cutters. The center of their activities is the fungus garden, which is also the site of the queen and brood. The garden, in most species, is made from fresh green leaves or other vegetal material. The ants forage for this, forming distinct trails to the vegetation that is being harvested. The cut leaves or other substrate are brought into the nest and prepared for the fungus. Fresh leaves and flowers are cut into pieces a millimeter or two in diameter; the ants form them into a pulpy mass by pinching them with the mandibles and adding saliva. Anal droplets are deposited on the pieces, which are then forced into place in the garden. Planting of the fungus is accomplished by an ant's picking up tufts of the adjacent mycelium and dotting the surface of the new substrate with it. The combination of salivary and anal secretions, together with the constant care given by the ants, facilitates the growth of the ant fungus only, despite constant possibilities for contamination. When the ants are removed, alien fungi and other organisms flourish. A mature nest of Atta Sexdens may consist of 2000 chambers, some temporarily empty, some with refuse, and the remainder with fungus gardens. Thousands of kilograms of fresh leaves will have been used. A young laboratory colony of Atta cephalotes will use 1 kilogram of fresh leaves for one garden. The attines are the chief agents for introducing organic matter into the soil in tropical rain forests; this matter becomes the nucleus for a host of other organisms, including nematodes and arthropods, after it is discarded by the ants. One ant species cultures a yeast; all others grow a mycelium. In the higher species the mycelium forms clusters of inflated hyphae. Mycologists accept as valid two names for confirmed fruiting stages: Leucocoprinus ( or Leucoagaricus) gongylophora (Moeller 1893) and Lepiota n. sp.