Abstract
Reduced expression or loss of tumor suppressor genes play a key role in many cancers. In this study, we investigated the role of RASSF1A in the pathogenesis of esophageal squamous cell carcinoma (ESCC) and nasopharyngeal carcinoma (NPC). We detected the down-regulated expression of both RASSF1A transcripts and protein in tumor tissues using RT-PCR and tissue microarray immunohistochemical staining analyses. Down-regulated expression of RASSF1A showed a significant association with WHO grade, tumor status, and lymph node metastasis, showing its possible utility as a biomarker for clinical specimens.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, N.I.H., Intramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
Aged
-
Blotting, Western
-
Carcinoma, Squamous Cell / genetics
-
Carcinoma, Squamous Cell / metabolism
-
Carcinoma, Squamous Cell / pathology*
-
Esophageal Neoplasms / genetics
-
Esophageal Neoplasms / metabolism
-
Esophageal Neoplasms / pathology*
-
Female
-
Gene Expression Regulation, Neoplastic*
-
Humans
-
Immunohistochemistry
-
Male
-
Middle Aged
-
Nasopharyngeal Neoplasms / genetics
-
Nasopharyngeal Neoplasms / metabolism
-
Nasopharyngeal Neoplasms / pathology*
-
Neoplasm Staging
-
Reverse Transcriptase Polymerase Chain Reaction
-
Tissue Array Analysis
-
Tumor Suppressor Proteins / genetics*
-
Tumor Suppressor Proteins / metabolism
Substances
-
RASSF1 protein, human
-
Tumor Suppressor Proteins