Application of the hsp65 PRA method for the rapid identification of mycobacteria isolated from clinical samples in Belgium

J Microbiol Methods. 2007 Oct;71(1):39-43. doi: 10.1016/j.mimet.2007.07.008. Epub 2007 Aug 1.

Abstract

Biochemical identification of mycobacteria is slow and many times fail to produce correct results. We compared PCR-restriction fragment length polymorphism analysis (PRA) of hsp65 and biochemical methods for the identification of mycobacteria from human samples in Belgium. PRA was found useful in the identification of mycobacteria and simple to implement as a quick method in the laboratory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques*
  • Belgium
  • Chaperonin 60
  • Chaperonins / genetics*
  • DNA, Bacterial / analysis
  • Humans
  • Mycobacterium / classification
  • Mycobacterium / genetics
  • Mycobacterium / isolation & purification*
  • Mycobacterium Infections / diagnosis*
  • Mycobacterium Infections / microbiology
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Restriction Fragment Length
  • Tuberculosis / microbiology

Substances

  • Bacterial Proteins
  • Chaperonin 60
  • DNA, Bacterial
  • heat-shock protein 65, Mycobacterium
  • Chaperonins