Objective: To evaluate the long-term stability of B-type natriuretic peptide (BNP) in frozen samples at -20 degrees C.
Design and methods: During a health and nutrition survey of a representative sample of the adult population of Porto, Portugal, blood samples were collected in EDTA tubes. Aliquots of plasma were frozen at -20 degrees C until measurement. Subjects with cardiac structural or functional abnormalities or renal dysfunction were excluded. BNP was assessed using a commercial immunofluorescence assay (Triage BNP Test) as a continuous variable (n=340) and in four subgroups selected according to different storage periods: < or =1 month (n=35), 6-12 months (n=80), 20-24 months (n=18) and > or =36 months (n=21).
Results: Age- and sex-adjusted BNP values, analyzed as a continuous variable, decreased significantly over time of storage. When we stratified in four intervals of storage time, the median BNP value (25th-75th percentile, p value) was: 22.6 pg/mL (8.9-76.2, reference group) for < or =1 month, 19.8 pg/mL (9.5-48.4, p=0.055) for 6-12 months, 15.3 pg/mL (7.5-35.0, p=0.037) for 20-24 months, and 2.5 pg/mL (2.5-10.5, p<0.001) for > or =36 months.
Conclusions: BNP is stable at -20 degrees C for 1 year, without protease inhibitors.