High-performance liquid chromatographic method for direct separation of 5-(p-hydroxyphenyl)-5-phenylhydantoin enantiomers using a chiral tris(4-methylbenzoate) column

J Chromatogr. 1991 Jul 17;568(1):157-63. doi: 10.1016/0378-4347(91)80349-h.

Abstract

After simple purification of the incubation mixture of phenytoin in isolated rat hepatocytes, 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH), which formed as a major metabolite, was readily resolved to each enantiomer by direct high-performance liquid chromatography on a cellulose tris(4-methylbenzoate) column, with a mobile phase of ethanol-water. It was also observed that the formation of S-(-)-p-HPPH was dominant, and the S/R ratio was 11.5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzoates*
  • Chromatography, High Pressure Liquid / methods*
  • Liver / chemistry
  • Liver / cytology
  • Male
  • Phenytoin / analogs & derivatives*
  • Phenytoin / analysis
  • Rats
  • Rats, Inbred Strains

Substances

  • Benzoates
  • 5-(3-hydroxyphenyl)-5-phenylhydantoin
  • Phenytoin
  • methyl benzoate