Isolation and characterization of bacteriophage FC3-10 from Klebsiella spp

FEMS Microbiol Lett. 1991 Oct 15;67(3):291-7. doi: 10.1016/0378-1097(91)90491-r.

Abstract

FC3-10 is a Klebsiella spp. specific bacteriophage isolated on a rough mutant (strain KT707, chemotype Rd) of K. pneumoniae C3. The bacteriophage receptor for this phage was shown to be the low-molecular mass lipopolysaccharide (LPS) fraction (LPS-core oligosaccharides), specifically the heptose content of the LPS inner-core. This is the first phage isolated on Klebsiella, the receptor for which is the LPS-core. This phage was unable to plate on Salmonella typhimurium LPS mutants with chemotypes Rd2 or Re showing incomplete or no heptose content on their LPS-core, respectively. Spontaneous phage-resistant mutants from different Klebsiella strains were deep-rough LPS mutants or encapsulated revertants from unencapsulated mutant strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Surface / metabolism
  • Bacteriophages / isolation & purification*
  • Bacteriophages / metabolism
  • Bacteriophages / ultrastructure
  • Electrophoresis, Polyacrylamide Gel
  • Klebsiella*
  • Lipopolysaccharides / metabolism
  • Microscopy, Electron
  • Receptors, Virus / metabolism
  • Virus Activation

Substances

  • Antigens, Surface
  • Lipopolysaccharides
  • Receptors, Virus