Abstract
A multiplex PCR assay which combines detection of bacteria of the genus Listeria, Listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones I, II, and III of L. monocytogenes was developed. The assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Typing Techniques
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Clone Cells
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DNA, Bacterial / analysis*
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DNA, Bacterial / chemistry
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Disease Outbreaks
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Food Microbiology*
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Listeria monocytogenes / classification
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Listeria monocytogenes / genetics
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Listeria monocytogenes / isolation & purification*
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Listeriosis / epidemiology
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Listeriosis / microbiology*
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Polymerase Chain Reaction / methods*
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Serotyping