Population genetic data for 10 STRs included in the Humantype Chimera kit were obtained by multiplex PCR and subsequent automated fluorescent detection (ABI 310) from a sample of 220 unrelated individuals of Polish ancestry residing in north-eastern Poland. The genotype distributions conformed to HWE for all the analysed loci. The highly polymorphic systems exhibit a high informativeness and may be helpful in cases requiring an extension of the CODIS loci system, particularly in kinship analysis and deficiency cases.