Immobilization of Prussian Blue nanoparticles onto thiol SAM modified Au electrodes for electroanalytical or biosensor applications

J Nanosci Nanotechnol. 2007 Aug;7(8):2877-82. doi: 10.1166/jnn.2007.672.

Abstract

Poly(vinylpyrrolidone) (PVP)-protected Prussian Blue (PB) nanoparticles were prepared by simply mixing FeCI3 and K4Fe(CN)6 with absence or presence of HCI or/and KCI in water solution. The obtained PB nanoparticles were immobilized onto thiol self-assembled monolayer (SAM) modified Au electrodes. L-cysteine (Cys) and 1,8-octanedithiol (ODT) were compared as a bridge between the gold surface and the PB nanoparticles. The results show that PB prepared from the initial solution with KCI gives preferred electrochemical response and that Cys/Au shows improved immobilization effect of PB than ODT/Au. The obtained PB/Cys/Au electrodes exhibit electrocatalytic activity toward H2O2 reduction and DL-homocysteine (HCys) oxidation. Glucose oxidase (GOX) was immobilized onto PB modified electrode to explore the potentials for the design of oxidase-based biosensors. It is possible to anchor PB nanoparticles and develop their application on electroanalysis and biosensing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • Catalysis
  • Crystallization
  • Cysteine / chemistry
  • Electrochemistry / methods*
  • Electrodes
  • Ferrocyanides / chemistry*
  • Glucose Oxidase / chemistry
  • Gold / chemistry
  • Hydrogen Peroxide / chemistry
  • Nanoparticles / chemistry*
  • Oxygen / chemistry
  • Potassium Chloride / chemistry
  • Sulfhydryl Compounds / chemistry

Substances

  • Ferrocyanides
  • Sulfhydryl Compounds
  • Potassium Chloride
  • Gold
  • Hydrogen Peroxide
  • Glucose Oxidase
  • Cysteine
  • 1,8-octanedithiol
  • Oxygen
  • ferric ferrocyanide