Cyanobacteria as bioreactors for the synthesis of Au, Ag, Pd, and Pt nanoparticles via an enzyme-mediated route

J Nanosci Nanotechnol. 2007 Aug;7(8):2696-708. doi: 10.1166/jnn.2007.600.

Abstract

Common Anabaena, Calothrix, and Leptolyngbya cyanobacteria are shown to form Au, Ag, Pd, and Pt nanoparticles of well-controlled size. These nanoparticles are synthesized intra-cellularly, and naturally released in the culture medium, where they are stabilized by algal polysaccharides, allowing their easy recovery. The size of the recovered particles as well as the reaction yield is shown to depend on the cyanobacteria genus. Investigations of nanoparticle formation indicate that the intracellular nitrogenase enzyme is responsible for the metal reduction but that the cellular environment is involved in the colloid growth process.

MeSH terms

  • Bioreactors*
  • Biotechnology / methods*
  • Colloids / chemistry
  • Cyanobacteria / metabolism*
  • Enzymes / chemistry*
  • Gold / chemistry*
  • Metal Nanoparticles / chemistry*
  • Microscopy, Electron, Transmission
  • Nanoparticles / chemistry
  • Nitrogenase / chemistry
  • Palladium / chemistry*
  • Platinum / chemistry*
  • Polysaccharides / chemistry
  • Silver / chemistry*
  • X-Ray Diffraction

Substances

  • Colloids
  • Enzymes
  • Polysaccharides
  • Silver
  • Platinum
  • Palladium
  • Gold
  • Nitrogenase