Isolation of active yeast telomerase protein Est3p and investigation of its dimerization in vitro

Yu S Sharanov; E M Smekalova; M I Zvereva; O A Dontsova

doi:10.1134/s0006297907070036

Isolation of active yeast telomerase protein Est3p and investigation of its dimerization in vitro

Biochemistry (Mosc). 2007 Jul;72(7):702-6. doi: 10.1134/s0006297907070036.

Authors

Yu S Sharanov¹, E M Smekalova, M I Zvereva, O A Dontsova

Affiliation

¹ Chemical Faculty, Lomonosov Moscow State University, Moscow, 119992, Russia. sharanov@genebee.msu.ru

PMID: 17680761
DOI: 10.1134/s0006297907070036

Abstract

In this study we proposed a method for isolation of Est3p modified with various affinity tags, which is applicable for structural and functional studies, and investigated homo- and heterodimer formation with various recombinant forms of Est3p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dimerization
Fungal Proteins / isolation & purification*
Fungal Proteins / metabolism
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism

Substances

Fungal Proteins
Recombinant Proteins