Chemoenzymatic synthesis of feruloyl D-arabinose as a potential anti-mycobacterial agent

Christina Vafiadi; Evangelos Topakas; Luke J Alderwick; Gurdyal S Besra; Paul Christakopoulos

doi:10.1007/s10529-007-9440-4

Chemoenzymatic synthesis of feruloyl D-arabinose as a potential anti-mycobacterial agent

Biotechnol Lett. 2007 Nov;29(11):1771-4. doi: 10.1007/s10529-007-9440-4. Epub 2007 Aug 4.

Authors

Christina Vafiadi¹, Evangelos Topakas, Luke J Alderwick, Gurdyal S Besra, Paul Christakopoulos

Affiliation

¹ Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens, 15700, Greece.

PMID: 17676274
DOI: 10.1007/s10529-007-9440-4

Abstract

The feruloyl esterase (StFaeC) produced by Sporotrichum thermophile transfered the feruloyl group to D: -arabinose using a mixture of n-hexane, t-butanol and water. About 45% conversion of D: -arabinose to the feruloylated derivative was achieved. The compound had an MIC value against Mycobacterium bovis BCG of 25 microg/ml.

MeSH terms

Anti-Bacterial Agents / chemical synthesis*
Carboxylic Ester Hydrolases / isolation & purification
Carboxylic Ester Hydrolases / metabolism
Disaccharides / chemical synthesis*
Disaccharides / chemistry
Esterification
Mycobacterium bovis / drug effects

Substances

Anti-Bacterial Agents
Disaccharides
feruloyl arabinobiose
Carboxylic Ester Hydrolases
feruloyl esterase