2-Aminopyridine--a label for bridging of oligosaccharides HPLC profiling and glycoarray printing

N V Shilova; O E Galanina; A Yu Rubina; V I Butvilovskaya; M E Huflejt; J Chambers; A Roucoux; N V Bovin

doi:10.1007/s10719-007-9050-3

2-Aminopyridine--a label for bridging of oligosaccharides HPLC profiling and glycoarray printing

Glycoconj J. 2008 Jan;25(1):11-4. doi: 10.1007/s10719-007-9050-3. Epub 2007 Aug 4.

Authors

N V Shilova¹, O E Galanina, A Yu Rubina, V I Butvilovskaya, M E Huflejt, J Chambers, A Roucoux, N V Bovin

Affiliation

¹ Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.

PMID: 17674203
DOI: 10.1007/s10719-007-9050-3

Abstract

2-Aminopyridine derivatives of oligosaccharides (OS-AP) were printed onto microchips by two different ways. The first method is based on direct covalent insertion of OS-AP in polyacrylamide gel 3D chip. The second method is based on conversion of OS-AP into more reactive OS-aminoalditol followed by covalent printing onto NHS-activated glass slides. This approach extends the range of saccharides suitable for covalent printing due to availability of commercial OS-AP and easy high-performance liquid chromatography separation of glycoprotein N-chains in form of AP derivatives.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Aminopyridines / chemistry*
Antibodies / immunology
Chromatography, High Pressure Liquid / methods*
Glycomics / methods*
Humans
Microarray Analysis / methods*
Molecular Structure
Oligosaccharides / analysis*
Oligosaccharides / chemistry*

Substances

Aminopyridines
Antibodies
Oligosaccharides

Abstract

Publication types

MeSH terms

Substances

Grants and funding