Abstract
Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / cytology*
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Adipocytes / drug effects
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Adipocytes / physiology*
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Animals
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Proteins / administration & dosage*
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Bone Morphogenetic Proteins / genetics
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Bone Morphogenetic Proteins / metabolism
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Cell Differentiation / drug effects
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Cell Differentiation / physiology
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Cell Line
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Humans
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Mice
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Myoblasts / drug effects
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Myoblasts / physiology
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Osteoblasts / cytology*
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Osteoblasts / drug effects
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Osteoblasts / physiology*
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Osteogenesis / drug effects
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Osteogenesis / physiology
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Recombinant Proteins / administration & dosage
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Stem Cells / cytology*
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Stem Cells / drug effects
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Stem Cells / physiology*
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Transforming Growth Factor beta / administration & dosage*
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / metabolism
Substances
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BMP2 protein, human
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Bmp2 protein, mouse
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Proteins
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Recombinant Proteins
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Transforming Growth Factor beta