Human glyceraldehyde-3-phosphate dehydrogenase in man-rodent somatic cell hybrids

Science. 1976 Apr 2;192(4234):54-6. doi: 10.1126/science.176725.

Abstract

The human enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) forms heteropolymers with the rodent enzyme in man-rodent somatic cell hybrids. A gene specifying GAPDH is syntenic with the genes specifying the glycolytic enzymes triosephosphate isomerase (TPI) and lactate dehydrogenase B (LDH-B). The synteny of GAPDH, TPI, and LDH-B is the first evidence for the syntenic association of human genes that specify enzymes of the Embden-Meyerhof glycolytic pathway.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromosome Mapping
  • Chromosomes, Human, 6-12 and X*
  • Genes*
  • Genetic Linkage*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis*
  • Humans
  • Hybrid Cells / enzymology*
  • L-Lactate Dehydrogenase / biosynthesis
  • NAD / metabolism
  • Protein Conformation
  • Rodentia
  • Triose-Phosphate Isomerase / biosynthesis

Substances

  • NAD
  • L-Lactate Dehydrogenase
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Triose-Phosphate Isomerase