Abstract
The GltS Na+/glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate:Na+ symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and beta-galactosidase gene fusions generated by TnphoA, nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / analysis
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Alkaline Phosphatase / genetics
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Alkaline Phosphatase / metabolism
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Amino Acid Sequence
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Amino Acid Transport Systems, Acidic / analysis*
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Amino Acid Transport Systems, Acidic / genetics
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Amino Acid Transport Systems, Acidic / metabolism*
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Artificial Gene Fusion
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Cell Membrane / enzymology*
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Escherichia coli / enzymology*
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Escherichia coli Proteins / analysis*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Molecular Sequence Data
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Protein Biosynthesis*
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Recombinant Fusion Proteins / analysis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Symporters / analysis*
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Symporters / genetics
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Symporters / metabolism*
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beta-Galactosidase / analysis
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Amino Acid Transport Systems, Acidic
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Escherichia coli Proteins
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Recombinant Fusion Proteins
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Symporters
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gltS protein, E coli
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Alkaline Phosphatase
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beta-Galactosidase