Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters

Biophys J. 2007 Nov 1;93(9):3285-90. doi: 10.1529/biophysj.107.112201. Epub 2007 Jul 27.

Abstract

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Escherichia coli / cytology
  • Fluorescent Dyes / metabolism*
  • Macropodidae
  • Microscopy, Fluorescence / instrumentation*
  • Nanotechnology*

Substances

  • Fluorescent Dyes