Objective: To explore the quality and quantity of glutamate(Glu) on the auditory pathway of inferior colliculus when exposed to conditioning noise or traumatic sound to find the relationship between the change of neurotransmitters and the protection of hearing, and the mechanism in the phenomena.
Methods: Twenty Sprague-Dawley rats were divided into four groups: control group, conditioning group, intensive noise group and combining group. The hearing function was measured by ABR. Glu was detected by microdialysis and HPLC.
Results: The ABR levels after exposure were (47.0+/-2.7) dB SPL (intensive noise group ),(31.0+/-2.2) dB SPL (conditioning group ) and (36.0+/-2.2) dB SPL (combining group ).The levels of Glu were (310.5+/-78.5)x 10(-7)mol/L (intense noise group ),(162.9+/-64.1)x10(-7)mol/L (conditioning group ); (113.6+/-38.1)x10(-7)mol/L (combining group),(56.3+/-23.9)x10(-7)mol/L (control group).
Conclusion: The level of Glu on the auditory pathway of inferior colliculus was higher when exposed to noise . Glu was exhausted when given conditioning sound, and the level of Glu was lower when given following intense noise. High level of Glu can damage hearing, so exhaustion of Glu in sound condition may contribute to the protection.