Molecular cytogenetic analysis of complex karyotypes with derivative chromosome der(1)t(1;5) found in two patients with myeloid leukemia

Jela Melichercíková; Jana Brezinová; Zuzana Zemanová; Jaroslav Cermák; Kyra Michalová

doi:10.1016/j.cancergencyto.2007.05.001

Molecular cytogenetic analysis of complex karyotypes with derivative chromosome der(1)t(1;5) found in two patients with myeloid leukemia

Cancer Genet Cytogenet. 2007 Jul 15;176(2):150-5. doi: 10.1016/j.cancergencyto.2007.05.001.

Authors

Jela Melichercíková¹, Jana Brezinová, Zuzana Zemanová, Jaroslav Cermák, Kyra Michalová

Affiliation

¹ Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic.

PMID: 17656259
DOI: 10.1016/j.cancergencyto.2007.05.001

Abstract

Two patients with myeloid hematological malignancies were examined with conventional cytogenetic analysis, and complex chromosomal aberrations were found. Multicolor FISH (mFISH) was used to clarify these rearrangements. A similar translocation between chromosomes 1 and 5 was revealed in both patients. To specify breakpoints, high-resolution multicolor banding (mBAND) for chromosome 1 and chromosome 5 was performed. The breakpoints were resolved as der(1)t(1;5)(p21;p12) in patient 1 and as der(1)t(1;5)(p21;p11) in patient 2. The breakpoint on chromosome 1 was the same in both patients, in the 1p21 region. This region contains the colony stimulating factor 1 gene (CSF1), which may play a role in tumorigenesis of myeloid tissue.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Chromosomes, Human, Pair 1*
Chromosomes, Human, Pair 5*
Cytogenetic Analysis
Humans
Karyotyping
Leukemia, Myeloid / genetics*
Male
Middle Aged
Translocation, Genetic*
Tumor Cells, Cultured