Employing an inverted 'W' investigation procedure with 9 sampling locations and adopting a biodiversity analysis approach integrated with typical statistic method, this paper studied the effects of different long-term stationary fertilization regimes on the weed biodiversity in winter wheat fields on loess soil. The results showed that in the experimental plots, there were 16 weed species belonging to 10 family and 16 genera, occupying about 34% of the total number of weed species in winter wheat fields in Shaanxi Province. The weed biodiversity was decreased with the improvement of soil nutrient status. There were 3-5 weed populations in treatments NPK and NPK plus organic materials, and 6-8 populations in treatments CK, N, NK and NP. The relative abundance of weeds ranged from 0 to 73%, and the ranges of Shannon's diversity index, Shannon's evenness index and Margalef' s species richness index were 0.2-1.08, 0.05-0.26 and 0.26-1.26, respectively. All of these 3 parameters were higher in unbalanced than in balanced fertilization treatments, and the differences between unbalanced and balanced fertilization treatments were significant in most case, which was probably due to the different status of available soil nutrients and might have different effects on the growth of weeds.