Abstract
Restriction fragment length polymorphism and DNA sequencing of polymerase chain reaction (PCR) products showed that a Lactobacillus rhamnosus strain of human origin resistant to macrolides, from which no resistance determinants have been detected by specific PCR and microarray screening, contained a heterozygous A-->G transition mutation at position 2058 (Escherichia coli numbering) of its 23S rRNA genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Drug Resistance, Bacterial / genetics*
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Gastrointestinal Tract / microbiology
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Genes, rRNA / genetics
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Humans
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Lacticaseibacillus rhamnosus / drug effects*
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Lacticaseibacillus rhamnosus / genetics*
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Macrolides / pharmacology*
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Molecular Sequence Data
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Point Mutation*
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 23S / genetics*
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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DNA, Ribosomal
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Macrolides
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RNA, Ribosomal, 23S
Associated data
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GENBANK/EF030190
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GENBANK/EF030191