Objective: To study the difference of rDNA ITS sequences between Cirtus grandis var. tomentosa and its relatives.
Methods: The rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced.
Results: The DNA sequences of ITS ranged from 675 to 683 bp and the 5.8S gene of all of the samples are 165 bp in size. Distance values of ITS sequence, estimated according to Kimura two-parameter models between C. grandis var. tomentosa and C. grandis var. shatin-you, C. grandis var. tomentosa and C. reticulata were 1.05 and 2.29 respectively.
Conclusion: There are some differences in rDNA ITS sequences among C. grandis var. tomentosa and its relatives.