Abstract
The role of endothelin (ET)B receptors in chemokine production in the brain of rats was examined. Intracerebroventricular administration of 500 pmol/day of Ala(1,3,11,15)-ET-1, a selective ETB agonist, for 3 or 7 days increased monocyte chemoattractant protein (MCP)-1 and cytokine-induced neutrophil chemoattractant (CINC)-1 mRNA in the caudate-putamen and cerebrum, whereas it had no effects on regulated on activation normal T-cell expressed and secreted (RANTES), fractalkine and stromal cell-derived factor (SDF)-1alpha mRNA expression. Immunoreactive MCP-1 and CINC-1 in the caudate-putamen and the cerebrum were increased by the ETB agonist. Immunohistochemical observations on the Ala(1,3,11,15)-ET-1-infused rats showed that glial fibrillary acidic protein-positive astrocytes had immunoreactivity for MCP-1 and CINC-1. These findings indicate that the activation of brain ETB receptors causes the production of MCP-1 and CINC-1, and suggest a pathophysiological role for brain ETB receptors in nervous system damage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Astrocytes / immunology
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Astrocytes / metabolism
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Brain / drug effects
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Brain / immunology
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Brain / metabolism*
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Chemokine CCL2 / genetics
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Chemokine CCL2 / immunology
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Chemokine CCL2 / metabolism*
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Chemokine CXCL1
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Chemokines, CXC / genetics
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Chemokines, CXC / immunology
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Chemokines, CXC / metabolism*
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Encephalitis / immunology
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Encephalitis / metabolism
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Encephalitis / physiopathology
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Endothelin-1 / analogs & derivatives
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Endothelin-1 / metabolism*
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Endothelins / pharmacology
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Glial Fibrillary Acidic Protein / metabolism
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Gliosis / immunology
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Gliosis / metabolism
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Gliosis / physiopathology
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Immunohistochemistry
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Injections, Intraventricular
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Male
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Neostriatum / drug effects
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Neostriatum / immunology
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Neostriatum / metabolism
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Receptor, Endothelin B / agonists
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Receptor, Endothelin B / metabolism*
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Telencephalon / drug effects
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Telencephalon / immunology
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Telencephalon / metabolism
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Up-Regulation / drug effects
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Up-Regulation / immunology
Substances
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Ccl2 protein, rat
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Chemokine CCL2
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Chemokine CXCL1
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Chemokines, CXC
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Cxcl1 protein, rat
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Endothelin-1
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Endothelins
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Glial Fibrillary Acidic Protein
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RNA, Messenger
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Receptor, Endothelin B
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endothelin 1, Ala(1,3,11,15)-