[Expression of EDA-fibronectin in injured human skin fibroblast and its potential application in estimation of wound healing]

Fa Yi Xue Za Zhi. 2007 Apr;23(2):94-6.
[Article in Chinese]

Abstract

Objective: [corrected] To study the expression of EDA-FN variant in injured human skin fibroblast and its relation to wound healing.

Methods: Human skin fibroblast cells were separated from human skin and cultured and monoclonal antibody to fibronectin [IST-9] was applied to amplify target segments.

Results: Trace amount of EDA-FN was detected in the culture cells. The expression was increased within 8 hours after the injury.

Conclusions: The expression of EDA-FN would be an ideal marker for estimation of wound healing

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cells, Cultured
  • Fibroblasts / metabolism*
  • Fibronectins / genetics
  • Fibronectins / metabolism*
  • Forensic Pathology
  • Humans
  • Immunohistochemistry
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Skin / cytology*
  • Time Factors
  • Wounds and Injuries*

Substances

  • Fibronectins
  • RNA, Messenger