Lariatins, novel anti-mycobacterial peptides with a lasso structure, produced by Rhodococcus jostii K01-B0171

J Antibiot (Tokyo). 2007 Jun;60(6):357-63. doi: 10.1038/ja.2007.48.

Abstract

Two anti-mycobacterial peptides with a lasso structure, named lariatins A and B, were separated by HP-20 and ODS column chromatographies and purified by HPLC from the culture broth of Rhodococcus jostii K01-B0171, which was isolated from soil aggregates collected in Yunnan, China. Lariains A and B showed growth inhibition against Mycobacterium smegmatis with MIC values of 3.13 and 6.25 microg/ml in agar dilution method, respectively. Furthermore, lariatin A inhibited the growth of Mycobacterium tuberculosis with an MIC of 0.39 microg/ml in liquid microdilution method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antitubercular Agents / chemistry
  • Antitubercular Agents / isolation & purification*
  • Antitubercular Agents / metabolism
  • Antitubercular Agents / pharmacology*
  • China
  • Fermentation
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Mycobacterium / drug effects
  • Peptides, Cyclic / chemistry
  • Peptides, Cyclic / isolation & purification*
  • Peptides, Cyclic / metabolism
  • Peptides, Cyclic / pharmacology*
  • Phylogeny
  • Polymerase Chain Reaction
  • Rhodococcus / chemistry*
  • Rhodococcus / classification
  • Rhodococcus / isolation & purification
  • Rhodococcus / metabolism
  • Soil Microbiology
  • Spectrometry, Mass, Fast Atom Bombardment
  • Spectrophotometry, Infrared
  • Spectrophotometry, Ultraviolet

Substances

  • Antitubercular Agents
  • Peptides, Cyclic
  • lariatin A
  • lariatin B