Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate

Appl Environ Microbiol. 2007 Sep;73(17):5676-8. doi: 10.1128/AEM.00278-07. Epub 2007 Jul 6.

Abstract

Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Biotechnology / methods*
  • Enzyme Stability
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Fructose / metabolism
  • Fructosediphosphates / biosynthesis*
  • Hot Temperature*
  • Phosphotransferases (Phosphate Group Acceptor) / genetics
  • Phosphotransferases (Phosphate Group Acceptor) / metabolism*
  • Polyphosphates / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*
  • Thermus thermophilus / enzymology
  • Thermus thermophilus / genetics

Substances

  • Fructosediphosphates
  • Polyphosphates
  • Recombinant Proteins
  • Fructose
  • Adenosine Triphosphate
  • Phosphotransferases (Phosphate Group Acceptor)
  • polyphosphate kinase
  • fructose-1,6-diphosphate