Abstract
Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of fructose 1,6-diphosphate from fructose and polyphosphate.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / biosynthesis*
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Biotechnology / methods*
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Enzyme Stability
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Fructose / metabolism
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Fructosediphosphates / biosynthesis*
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Hot Temperature*
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Phosphotransferases (Phosphate Group Acceptor) / genetics
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Phosphotransferases (Phosphate Group Acceptor) / metabolism*
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Polyphosphates / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism*
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Thermus thermophilus / enzymology
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Thermus thermophilus / genetics
Substances
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Fructosediphosphates
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Polyphosphates
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Recombinant Proteins
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Fructose
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Adenosine Triphosphate
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Phosphotransferases (Phosphate Group Acceptor)
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polyphosphate kinase
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fructose-1,6-diphosphate