Toxic fungal metabolites - mycotoxins - cause poisonings after consumption of contaminated food commodities. The most probable intoxications are connected with eating poorly stored food or inhaling of moldy dust. One of the effective ways to protect people against mycotoxins is timely detection. Several methods such as affinity chromatography and enzyme-linked immunosorbent assay are commercially available for this purpose. Nevertheless, fast, sensitive, simple, portable, and low-cost devices are difficult to find. Application of biosensors appears to be a possible method to meet this need for mycotoxins assay.