Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens

J Clin Microbiol. 2007 Sep;45(9):3089-94. doi: 10.1128/JCM.00858-07. Epub 2007 Jul 3.

Abstract

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / methods*
  • Biopsy
  • Helicobacter Infections / diagnosis
  • Helicobacter Infections / microbiology*
  • Helicobacter Infections / pathology
  • Helicobacter pylori / genetics
  • Helicobacter pylori / isolation & purification*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Peptide Nucleic Acids*
  • Sensitivity and Specificity
  • Stomach / microbiology*
  • Stomach / pathology

Substances

  • Peptide Nucleic Acids