A novel method for producing electron transparent films of interfaces between cells and biomaterials

J Mater Sci Mater Med. 2008 Jan;19(1):467-70. doi: 10.1007/s10856-006-0042-9. Epub 2007 Jul 3.

Abstract

Transmission electron microscopy (TEM) investigations of intact interfaces of cells and brittle biomaterials have proven difficult using common TEM preparation techniques. This paper describes a technique to fabricate thin sections for TEM investigation of intact interfaces between human monocytes and sintered hydroxylapatite by the use of focused ion beam (FIB) microscopy. The interfaces were examined using energy filtered TEM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials / chemistry*
  • Coated Materials, Biocompatible
  • Electrons
  • Equipment Design
  • Humans
  • Ions
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Osseointegration
  • Surface Properties
  • Tissue Engineering / methods*
  • U937 Cells

Substances

  • Biocompatible Materials
  • Coated Materials, Biocompatible
  • Ions