Recently, the capsule endoscope has been highlighted for the patient's convenience and the possibility of application in the small intestine. However, the capsule endoscope has some limitations in obtaining an image of the digestive organ because its movement depends only on the peristaltic motion. In order to solve these problems, it is necessary to determine the locomotive mechanism of the capsule endoscope. Therefore, the present authors have already proposed an earthworm-like robot, which has a locomotive mechanism. However, this mechanism should be designed so that the earthworm-like robot has a larger stroke than the critical stroke required to perform motion inside the small intestine. In this study, therefore, not only is the modelling of the locomotive process based on a biomechanical study presented but also the movement of the earthworm-like robot in the small intestine is simulated. Through the simulation process, the variation in the critical stroke with regard to the elastic modulus of the mesentery is investigated. Finally, from an in vitro test of the proposed robot, it is found that the experimental result is very similar to that of the simulation. Consequently, the present work will provide guidelines for designing an earthworm-like robot for diagnosis of the small intestine.