We explore psychometric characteristics of a modified version of the Cloninger's personality questionnaire, the Temperament and Character Inventory-Revised (TCI-R) in a 200-subject sample. This sample was stratified in accordance with the Czech population. We performed principal component analyses and explored the factorial structure of the questionnaire, to establish internal consistency of each dimension. The factorial structure of the TCI-R was well defined for temperament, but not for character. A robust factor was obtained for Persistence. All dimensions obtained higher alpha Cronbach coefficients with the TCI-R than with the TCI. There were high reliability coefficients in test-retest for TCI-R and lower for TCI/TCI-R were found. Significant correlations were obtained between age and NS, RD and SD. The TCI-R seems to have similar psychometric characteristics to TCI, with significant improvements in temperament factor structure and internal consistency of most dimensions.