The purpose of this study was to compare osteoporosis health beliefs among different age and gender groups. This study used a cross-sectional design, involved 300 participants that represent both genders and three age groups (18 to 25, 30 to 50, and 50-plus), and assessed osteoporosis health beliefs using the Osteoporosis Health Belief Scale (OHBS). Factor analysis revealed three subscales of OHBS: perceived susceptibility, seriousness of the condition, and health motivation to take preventative actions with acceptable levels of reliability. Significant age and gender differences were observed in the susceptibility scores of the OHBS. The perceived seriousness of the osteoporosis scores was similar across all age and gender groups, as was health motivation. These results highlight the need for osteoporosis awareness programs targeting different aspects of belief perceptions, specifically for younger women and men.