Identification of the major protein component of the pneumococcal eclipse complex

J Bacteriol. 2007 Sep;189(17):6497-500. doi: 10.1128/JB.00687-07. Epub 2007 Jun 29.

Abstract

During genetic transformation of Streptococcus pneumoniae, single strands from native donor DNA enter competent cells, where they associate with an unidentified protein with a molecular mass of 15 to 20 kDa to form the eclipse complex. Using Western blotting, we identify the principal protein cofractionating with donor DNA in this complex as SsbB.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / isolation & purification*
  • Blotting, Western
  • DNA-Binding Proteins / isolation & purification*
  • Molecular Weight
  • Streptococcus pneumoniae / chemistry*

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins