Identification of trehalose dimycolate (cord factor) in Mycobacterium leprae

FEBS Lett. 2007 Jul 24;581(18):3345-50. doi: 10.1016/j.febslet.2007.06.029. Epub 2007 Jun 21.

Abstract

Glycolipids of Mycobacterium leprae obtained from armadillo tissue nodules infected with the bacteria were analyzed. Mass spectrometric analysis of the glycolipids indicated the presence of trehalose 6,6'-dimycolate (TDM) together with trehalose 6-monomycolate (TMM) and phenolic glycolipid-I (PGL-I). The analysis showed that M. leprae-derived TDM and TMM possessed both alpha- and keto-mycolates centering at C78 in the former and at C81 or 83 in the latter subclasses, respectively. For the first time, MALDI-TOF mass analyses showed the presence of TDM in M. leprae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / chemistry
  • Chromatography, Thin Layer
  • Cord Factors / chemistry*
  • Cord Factors / isolation & purification*
  • Cord Factors / metabolism
  • Esters / chemistry
  • Glycolipids / chemistry
  • Methylation
  • Molecular Structure
  • Mycobacterium leprae / chemistry*
  • Mycobacterium leprae / metabolism*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Antigens, Bacterial
  • Cord Factors
  • Esters
  • Glycolipids
  • phenolic glycolipid I, Mycobacterium leprae