Abstract
This study was undertaken to identify cellular proteins that bind an orally active natural product insulin mimic. Phage display cloning was used with a biotinylated derivative of this molecule as bait. Among the proteins identified was glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which has recently been shown to affect insulin receptor signaling. Binding data support a role for human GAPDH as another target of the insulin mimic, which could explain its action as a selective insulin receptor modulator.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Bacteriophage T7 / metabolism
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Binding Sites
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Erythrocytes / enzymology
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Glyceraldehyde-3-Phosphate Dehydrogenases / chemistry*
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Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
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Humans
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Indoles / chemistry*
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Indoles / pharmacology
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Insulin / physiology*
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Molecular Mimicry
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Molecular Sequence Data
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Peptide Library
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Surface Plasmon Resonance
Substances
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Indoles
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Insulin
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Peptide Library
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Glyceraldehyde-3-Phosphate Dehydrogenases
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demethylasterriquinone A1