Abstract
According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
MeSH terms
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Biocatalysis
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Blotting, Western
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Cloning, Molecular
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Electrophoresis, Polyacrylamide Gel
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Fungal
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Glucan 1,4-alpha-Glucosidase / genetics*
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Glucan 1,4-alpha-Glucosidase / metabolism
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Molecular Sequence Data
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Pichia / genetics
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Recombinant Proteins / metabolism
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Rhizopus / enzymology
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Rhizopus / genetics*
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Sequence Analysis, DNA
Substances
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Fungal Proteins
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Recombinant Proteins
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Glucan 1,4-alpha-Glucosidase