Polysaccharides in Several traditional Chinese medicine compounds of Dioscorea bulbifera L, Dioscorea bulbifera L and Chinese Angelica compounds (3: 2 by mass ratio), Hedyotis diffusa Willd and Scutellrla barbata D. Don compounds (3: 2 by mass ratio) were extracted by ultrasound and the content of polysaccharides were determined by the colorimetry. Dioscorea bulbifera L and Chinese Angelica compounds contained the biggest polysaccharide, 16. 509%. All of these three kinds of polysaccharide had anti-tumor activity. The anti-tumor activity was Dioscorea bulbifera L and Chinese Angelica compounds > Hedyotis diffusa Willd and Scutellrla barbata D. Don compounds > Dioscorea bubifera L.