Previous work by Bryden and Roy (1999) showed a larger performance difference between the hands for placing pegs into holes on the Grooved Pegboard test than for removing pegs from the holes. The authors argued that these data provided evidence of the importance of task demands in manual asymmetries. However the study failed to control for the differing starting positions of the pegs. To clarify this possible confound, the purpose of the current investigation was to determine the influence of starting position on the between-hand performance differences on the Grooved Pegboard Test. To do so, both the start and end positions were manipulated, such that participants moved the pegs from the receptacle to another receptacle or to a set of holes, or participants moved the pegs from a set of holes to a receptacle or another set of holes. A total of 30 right-handed individuals (as classified using the Waterloo Handedness Questionnaire) participated in the experiment, completing five trials with each hand for each of the four conditions. While no significant effects of start position were found, a significant interaction between hand and end position, F(1.29) = 30.85, P<.001, was found for the time to complete the task, where larger differences between the hands, fovouring the right hand, were seen for placing pegs into the holes as opposed to the receptacles. This effect was also found when the data were expressed using a laterality quotient. The results are discussed in terms of the influence of task complexity on manual asymmetries.