Relationship between vitamin D binding protein and aspirin resistance in coronary ischemic patients: a proteomic study

J Proteome Res. 2007 Jul;6(7):2481-7. doi: 10.1021/pr060600i. Epub 2007 Jun 8.

Abstract

Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of the fibrinogen gamma chain and three isotypes of haptoglobin was increased in ASA-resistant patients. Three vitamin D binding protein isotypes were increased in ASA-resistant patients. In vitro incubation of vitamin D binding protein (DBP) with blood from healthy volunteers reduced the inhibitory effect of ASA on thromboxane A2 production. DBP may be a new regulator of the inhibitory effect of ASA on platelets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aspirin / pharmacology
  • Aspirin / therapeutic use*
  • Blood Proteins / analysis
  • Blood Proteins / metabolism
  • Blood Proteins / pharmacology
  • Coronary Disease / blood*
  • Coronary Disease / drug therapy
  • Coronary Disease / metabolism
  • Drug Resistance*
  • Electrophoresis, Gel, Two-Dimensional
  • Female
  • Humans
  • Male
  • Middle Aged
  • Platelet Aggregation / drug effects
  • Platelet Aggregation Inhibitors / pharmacology
  • Protein Isoforms / blood
  • Proteome / analysis*
  • Proteome / metabolism
  • Proteomics
  • Thromboxane A2 / antagonists & inhibitors
  • Thromboxane A2 / metabolism
  • Vitamin D-Binding Protein / blood*
  • Vitamin D-Binding Protein / metabolism
  • Vitamin D-Binding Protein / pharmacology

Substances

  • Blood Proteins
  • Platelet Aggregation Inhibitors
  • Protein Isoforms
  • Proteome
  • Vitamin D-Binding Protein
  • Thromboxane A2
  • Aspirin