Abstract
Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of the fibrinogen gamma chain and three isotypes of haptoglobin was increased in ASA-resistant patients. Three vitamin D binding protein isotypes were increased in ASA-resistant patients. In vitro incubation of vitamin D binding protein (DBP) with blood from healthy volunteers reduced the inhibitory effect of ASA on thromboxane A2 production. DBP may be a new regulator of the inhibitory effect of ASA on platelets.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Aspirin / pharmacology
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Aspirin / therapeutic use*
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Blood Proteins / analysis
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Blood Proteins / metabolism
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Blood Proteins / pharmacology
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Coronary Disease / blood*
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Coronary Disease / drug therapy
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Coronary Disease / metabolism
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Drug Resistance*
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Electrophoresis, Gel, Two-Dimensional
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Female
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Humans
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Male
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Middle Aged
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Platelet Aggregation / drug effects
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Platelet Aggregation Inhibitors / pharmacology
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Protein Isoforms / blood
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Proteome / analysis*
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Proteome / metabolism
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Proteomics
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Thromboxane A2 / antagonists & inhibitors
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Thromboxane A2 / metabolism
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Vitamin D-Binding Protein / blood*
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Vitamin D-Binding Protein / metabolism
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Vitamin D-Binding Protein / pharmacology
Substances
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Blood Proteins
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Platelet Aggregation Inhibitors
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Protein Isoforms
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Proteome
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Vitamin D-Binding Protein
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Thromboxane A2
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Aspirin