Abstract
Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription-quantitative PCRs (RT-qPCRs) that amplified 2 different segments of bluetongue virus detected this exotic strain. These 2 RT-qPCRs detected infection earlier than a competitive ELISA for antibody detection.
MeSH terms
-
Animals
-
Antibodies, Viral / blood
-
Belgium / epidemiology
-
Bluetongue virus* / genetics
-
Bluetongue virus* / immunology
-
Bluetongue virus* / isolation & purification
-
Bluetongue virus* / pathogenicity
-
Bluetongue* / diagnosis
-
Bluetongue* / epidemiology
-
Bluetongue* / physiopathology
-
Bluetongue* / virology
-
Cattle
-
Cattle Diseases* / diagnosis
-
Cattle Diseases* / epidemiology
-
Cattle Diseases* / physiopathology
-
Cattle Diseases* / virology
-
Cell Line
-
Cricetinae
-
Enzyme-Linked Immunosorbent Assay
-
RNA, Viral / blood
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sheep / virology*
-
Viral Load
Substances
-
Antibodies, Viral
-
RNA, Viral