Abstract
Calcium Ruby m-Cl (X = H, Y = Cl) is a visible-light excited red-emitting calcium concentration ([Ca2+]) indicator dye (579/598 nm peak excitation/emission) with a side arm for conjugation via EDC or click chemistry. Its large molar extinction and high quantum yield rank it among the brightest long-wavelength Ca2+ indicators. Calcium Ruby is a promising alternative to existing dyes for imaging [Ca2+] in multicolor fluorescence applications or in the presence of yellow-green cellular autofluorescence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Calcium / chemistry*
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Chelating Agents / chemical synthesis
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Chelating Agents / chemistry
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Egtazic Acid / analogs & derivatives
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Egtazic Acid / chemical synthesis
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Egtazic Acid / chemistry
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Electrochemistry
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Fluorescence
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Indicators and Reagents / chemical synthesis
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Indicators and Reagents / chemistry*
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Spectrophotometry, Infrared
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Trifluoroacetic Acid / chemistry
Substances
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Chelating Agents
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Indicators and Reagents
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Egtazic Acid
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Trifluoroacetic Acid
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1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
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Calcium