Synthesis and characterization of a new red-emitting Ca2+ indicator, calcium ruby

Org Lett. 2007 Jul 5;9(14):2629-32. doi: 10.1021/ol070648h. Epub 2007 Jun 7.

Abstract

Calcium Ruby m-Cl (X = H, Y = Cl) is a visible-light excited red-emitting calcium concentration ([Ca2+]) indicator dye (579/598 nm peak excitation/emission) with a side arm for conjugation via EDC or click chemistry. Its large molar extinction and high quantum yield rank it among the brightest long-wavelength Ca2+ indicators. Calcium Ruby is a promising alternative to existing dyes for imaging [Ca2+] in multicolor fluorescence applications or in the presence of yellow-green cellular autofluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / chemistry*
  • Chelating Agents / chemical synthesis
  • Chelating Agents / chemistry
  • Egtazic Acid / analogs & derivatives
  • Egtazic Acid / chemical synthesis
  • Egtazic Acid / chemistry
  • Electrochemistry
  • Fluorescence
  • Indicators and Reagents / chemical synthesis
  • Indicators and Reagents / chemistry*
  • Spectrophotometry, Infrared
  • Trifluoroacetic Acid / chemistry

Substances

  • Chelating Agents
  • Indicators and Reagents
  • Egtazic Acid
  • Trifluoroacetic Acid
  • 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
  • Calcium